Journal: The American Journal of Tropical Medicine and Hygiene

Article Title: A Phase II, Randomized, Safety and Immunogenicity Study of a Re-Derived, Live-Attenuated Dengue Virus Vaccine in Healthy Adults

doi: 10.4269/ajtmh.2012.12-0361

Figure Lengend Snippet: Mono-, bi-, tri-, and tetravalent responses to DENV types in unprimed subjects per group and dose (ATP cohort). Percentages of initially unprimed subjects in each group (F17/Pre, F17, and F19) having seroconverted for none (grey), one (blue), two (green), three (red), or four (orange) DENV types after each dose of the candidate vaccine are shown. Blood samples were taken from subjects 1 month after each dose.

Article Snippet: – The Walter Reed Army Institute of Research (WRAIR) in collaboration with GlaxoSmithKline Vaccines (GSK) developed a live-attenuated tetravalent dengue virus vaccine candidate comprised of four live virus strains representing each of the four DENV types attenuated by serial passage in primary dog kidney (PDK) cells., A safe, well-tolerated, and immunogenic preparation of the vaccine candidate was identified in a phase II trial conducted in the United States in adult subjects.

Techniques:

Journal: The Korean Journal of Parasitology

Article Title: Ignatzschineria larvae Bacteremia Following Lucilia sp. Myiasis in an Irregular Migrant: A Case Report

doi: 10.3347/kjp.2021.59.2.159

Figure Lengend Snippet: Ignatzschineria larvae from pure bacterial culture: Gram stain.

Article Snippet: Recent inclusion of reference spectra for a representative strain of Ignatzschineria indica in the Bruker database may make possible more frequent identification of this species by MALDI-TOF MS in the future [ , ].

Techniques: Staining

Journal: The Korean Journal of Parasitology

Article Title: Ignatzschineria larvae Bacteremia Following Lucilia sp. Myiasis in an Irregular Migrant: A Case Report

doi: 10.3347/kjp.2021.59.2.159

Figure Lengend Snippet: Culture of Ignatzschineria larvae grown on (A) Columbia agar and (B) chocolate agar after 18-hour incubation.

Article Snippet: Recent inclusion of reference spectra for a representative strain of Ignatzschineria indica in the Bruker database may make possible more frequent identification of this species by MALDI-TOF MS in the future [ , ].

Techniques: Chocolate, Incubation

Journal: The Korean Journal of Parasitology

Article Title: Ignatzschineria larvae Bacteremia Following Lucilia sp. Myiasis in an Irregular Migrant: A Case Report

doi: 10.3347/kjp.2021.59.2.159

Figure Lengend Snippet: Neighbour-Joining phylogenetic tree based on nearly full-length 16S rRNA gene sequence of Ignatzschineria larvae isolate BM1043 from our patient (in boldface) and representative Ignatzschineria sp. sequences retrieved from GenBank. The tree was created with MEGA 7.0 software using the Neighbour-Joining method and Jukes–Cantor model. Values on branches are percentage bootstrap values using 1,000 replicates. Wohlfahrtiimonas chitiniclastica was included as an outgroup organism.

Article Snippet: Recent inclusion of reference spectra for a representative strain of Ignatzschineria indica in the Bruker database may make possible more frequent identification of this species by MALDI-TOF MS in the future [ , ].

Techniques: Sequencing, Software

Journal: Vaccines

Article Title: Flublok Quadrivalent Vaccine Adjuvanted with R-DOTAP Elicits a Robust and Multifunctional CD4 T Cell Response That Is of Greater Magnitude and Functional Diversity Than Conventional Adjuvant Systems

doi: 10.3390/vaccines12030281

Figure Lengend Snippet: Mapping of H3 CD4 T cell epitopes in B6 mice. Shown in ( A ) is peptide matrix, with all peptides that collectively span H3 ( H3N2 Perth2009 ) contained in discreet pools of 9-10 peptides indicated by R1 though R10 or C1 through C10. After priming B6 mice by infection with A/Switzerland 2013 H3N2 at 900 PFU/mouse, day 12 post infection CD4 T cells isolated from the spleen were tested for recognition of the peptides in each pool using a cytokine EliSpot assay ( B ) for IL-2 (top) or IFN-γ (bottom). Peptides in pools C8 and R10 were the most stimulatory followed by C7 and R9. The candidate peptides are highlighted in ( A ), with the major candidates boxed in red outline. Peptide 18 and 50 were eliminated in a subsequent assay, shown in ( C ) in IL-2 (left) and IFN-γ (right) EliSpots. Shown in ( D ), red box, is the likely core of the dominant H3 peptide, contained primarily in peptide 36.

Article Snippet: An Influenza A H3 A/Perth/09 peptide array containing overlapping 15-mer peptides was obtained from BEIR (BEI Resources, NIAID, Bethesda, MD, NR-19266), and single peptides (17-mers) representing divergent sequences from recently circulating H3 strains were purchased from Genscript (Piscataway, NJ, USA).

Techniques: Infection, Isolation, Enzyme-linked Immunospot, Subsequent Assay

Journal: Vaccines

Article Title: Flublok Quadrivalent Vaccine Adjuvanted with R-DOTAP Elicits a Robust and Multifunctional CD4 T Cell Response That Is of Greater Magnitude and Functional Diversity Than Conventional Adjuvant Systems

doi: 10.3390/vaccines12030281

Figure Lengend Snippet: Cytokine EliSpot assays test the specificity of CD4 T cells elicited by Flublok adjuvanted with R-DOTAP. Mice were vaccinated with Flublok adjuvanted with R-DOTAP in the rear footpad and at day 9 the draining lymph node cells were enriched for CD4 T cells. Candidate peptides or peptide pools were tested for their ability to stimulate the CD4 T cells for production of IL-2 ( left ) or IFN-γ ( right ). The points represent the data from two independent assays and the average is shown the by the grey bar. H3N+ is a pool of peptides from the amino terminal half of the H3 Perth 2009 with added H3 strain variant peptides and the H3C+ is the comparable pool of peptides from the COOH terminus of H3, demonstrating that the defined H3 35/36 peptide comprises the primary epitope specificity. Also shown are the CD4 T cell responses to the 3 HA-B peptides previously defined.

Article Snippet: An Influenza A H3 A/Perth/09 peptide array containing overlapping 15-mer peptides was obtained from BEIR (BEI Resources, NIAID, Bethesda, MD, NR-19266), and single peptides (17-mers) representing divergent sequences from recently circulating H3 strains were purchased from Genscript (Piscataway, NJ, USA).

Techniques: Enzyme-linked Immunospot, Variant Assay